Figure 5. Absence of reaction by in situ hybridization (ISH) to the digoxigenin (DIG)-labeled Taura syndrome virus (TSV) probes within the BGMK cells harvested at day 7 postinjection with TSV. A) No cytopathic effect suggestive of TSV infection was evident by conventional hematoxylin/eosin-phloxin (H&E) histology (H&E stain; 100x). B) Consecutive histologic section to that shown in 4A, but subjected to ISH with DIG-labeled TSV probes specific for TSV. No reaction to TSV is apparent in the challenged cells (Bismarck Brown counterstain; 100x).
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