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Volume 10, Number 12—December 2004

Research

Nonsusceptibility of Primate Cells to Taura Syndrome Virus

Carlos R. Pantoja*Comments to Author , Solangel A. Navarro*, Jaime Naranjo*, Donald V. Lightner*, and Charles P. Gerba*
Author affiliations: *University of Arizona, Tucson, Arizona, USA

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Table

Mean TSV quantification in tissue cell culture mediaa,b

Cell type Source of inoculum Inoculum type Mean viral quantification (viral copies/μL) postexposure
Day 0 Day 4 Day 7
BGMK Hemolymph TSV-infected 2.7 x 106 2.4 x 105 2.5 x 104
SPFc 0 0 0
Hepatopancreas TSV-infected 1.2 x 104 5.0 x 103 5.0 x 103
SPF 0 0 0
FrhK-4 Hemolymph TSV-infected 1.3 x 105 1.9 x 104 4.7 x 103
SPF 0 0 0
Hepatopancreas TSV-infected 1.3 x 104 8.4 x 103 3.7 x 103
SPF 0 0 0
MA-104 Hemolymph TSV-infected 1.5 x 105 3.1 x 104 2.3 x 103
SPF 0 0 0
Hepatopancreas TSV-infected 1.2 x 104 1.3 x 104 9.2 x 103
SPF 0 0 0

aEstimated by real time reverse transcription–polymerase chain reaction at different intervals postexposure.
bBGMK, Buffalo green monkey kidney; FrhK-4, monkey Rhesus female kidney embryonic; MA-104, monkey African green kidney; TSV; Taura syndrome virus; SPF, specific pathogen free.
cInoculum originated from SPF penaeid shrimp.

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