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Volume 10, Number 3—March 2004

Dispatch

Endemic Carbapenem-resistant Pseudomonas aeruginosa with Acquired Metallo-β-lactamase Determinants in European Hospital

Cristina Lagatolla*Comments to Author , Enrico A. Tonin*, Carlo Monti-Bragadin*, Lucilla Dolzani*, Francesca Gombac*, Claudia Bearzi*, Elisabetta Edalucci*, Fabrizia Gionechetti*, and Gian Maria Rossolini†
Author affiliations: *Università di Trieste, Trieste, Italy; †Università di Siena, Siena, Italy

Main Article

Table 1

Genetic relatedness, presence of MBL determinants, and distribution of the 89 imipenem-resistant Pseudomonas aeruginosa isolatesa

No. of isolates RAPD–AFLP genotypesb blaVIM allele Hospital wards (patients) Long-term care facilities (patients) Outpatients
blaVIM-positive





53
A
blaVIM-1-like
15 (43)
3 (4)
6
8
B
blaVIM-2-like
4c (8)
-
-
1
C
blaVIM-2-like
1d (1)
-
-
1
D
blaVIM-2-like
-
1 (1)
-
1
E
blaVIM-1-like
-
-
1
blaVIM-negative





5
A
None
2 (3)
-
2
1
B
None
1 (1)
-
-
19 F-G-H-I-J-Ke None 8 (16) 1 (1) 2

a MBL, metallo-β-lactamase.
bRAPD–AFLP, Random Amplification of Polymorphic DNA–Amplified Fragment Length Polymorphism. Results obtained with the two genotyping techniques were always consistent with each other.
cIn these wards isolates of cluster A were also detected.
dIn this ward isolates of clusters A and B were also detected.
eGenotypes F to K included a number of isolates ranging from 1 to 7.

Main Article

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