Candidaparapsilosis Characterization in an Outbreak Setting
Duncan M. Kuhn*1, Pranab K. Mukherjee*, Thomas A. Clark†, Claude Pujol‡, Jyotsna Chandra*, Rana A. Hajjeh†, David W. Warnock†, David R. Soll‡, and Mahmoud A. Ghannoum*
Author affiliations: *University Hospitals of Cleveland and Case Western Reserve University, Cleveland, Ohio, USA; †Centers for Disease Control and Prevention, Atlanta, Georgia, USA; ‡University of Iowa, Iowa City, Iowa, USA; 1Current affiliation: Division of Pulmonary and Critical Care, University of Washington Hospitals, Seattle, WA 98195, USA.
Figure 5. Secretory aspartic protease (SAP) expression by Candida parapsilosis clinical isolates. Panel A shows representative sodium dodecyl sulfate–polyacrylamide gel electrophoresis of various C. parapsilosis isolates. M, molecular weight marker lane; BSA, bovine serum albumin alone; other lanes show number of isolate; and +, supernatant plus protease inhibitor cocktail. Protease activity is evident from the appearance of lower molecular weight bands representing cleavage products. Thick arrow indicates the 20-kDa protein appearing after protease digestion. (For details of methods used see text.) Panel B shows densitometric scanning analysis of SAP activity. Strains 177 and 179 were included to demonstrate the heterogeneity in SAP production within the clonal strains. Cath, catheter; Bld, bloodstream; Spt, sputum; Hnd, hand; Wnd, wound; Pdf, peritoneal dialysis fluid.
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