Volume 10, Number 7—July 2004
Research
Molecular Analysis of Plasmodium ovale Variants
Thin Thida Win*, Amadu Jalloh*, Indah Setyawati Tantular†, Takafumi Tsuboi‡, Marcelo Urbano Ferreira§, Masatsugu Kimura¶, and Fumihiko Kawamoto*
Table 1
Oligonucleotide primers used in this study
| Target gene |
Primers |
Sequences (5′→3′) |
| A type of the SSUrRNA gene | 18S F | AACCTGGTTGATCTTGCCAGTAGTC |
| 18S F1 | CGATTCGGAGAGGGAGCCTGA | |
| PoR2 | TGAAGGAAGCAATCTAAGAAATTT | |
| P1F-UP | TCCATTAATCAAGAACGAAAGTTAAG | |
| 18S F2 | TGGATGGTGATGCATGGCCGT | |
| 18S R | TAATGATCCTTCCGCAGGTTCACC | |
| Cytochrome b gene | Cyt b 1F | ATGAATTATTATTCTATTAATTTAG |
| Cyt b 1R | GGATCACTTACAGTATATCCTCC | |
| Cyt b 2F | CAAATGAGTTATTGGGGTGCAAC | |
| Cyt b 2R | TTTTAACATTGCATAAAATGGTA | |
| Cyt b 3F | CCAAATCTATTAAGTCTTGATGT | |
| Cyt b 3R | TGTTTGCTTGGGAGCTGTAATCA | |
| Cysteine protease gene | CysP-F | GCCAGTGTAGGTAATATTGAAT |
| CysP-R | GTATAAAATATCATCATCATCA | |
| Ookinete surface protein genes | ||
| First polymerase chain reaction (PCR) | ||
| Pos 25 | Po8F2 | CTTTTGTTAGTATTTCCTCC |
| Po8R1C | ACATTGAACACAGAATATGC | |
| Pos 28-1 | Po1F1 | TCCCCTTTTGTCCGTTTGTC |
| Po1R1 | AAAGACTGCTACACGCATAC | |
| Pos 28-2 | Po4NF1 | GTTCATTACATTAAGTTCTC |
| Po4R1 | TTAAATTGTATAAATTACACTG | |
| Nested PCR | ||
| Pos 25 | Po8F1-in | TTACAGTTTGTTTCTCGTC |
| Po8R1-in | AGGTTTAAGACATTGAACAC | |
| Pos 28-1 | Po1F1-in | TTTTCTTTTCGTTTGCTTGC |
| Po1R1-in | TCAATATGGACACAGAATGC | |
| Pos 28-2 | Po4F1-in | TTTACCATTTTCCAATATGC |
| Po4R1-in | CAATTAAAATTAAAATTCTG |
