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Volume 10, Number 7—July 2004

Dispatch

SARS Coronavirus Detection

Andreas Nitsche*Comments to Author , Brunhilde Schweiger*, Heinz Ellerbrok*, Matthias Niedrig*, and Georg Pauli*
Author affiliations: *Robert Koch-Institut, Berlin, Germany

Main Article

Table 2

CT values and standard deviation of serial dilutions of SARS-CoV RNA subjected to different real-time RT-PCR assaysa

GE Real-time RT-PCR systems Artus kit
n = 4 Drosten et al. (5)
n = 6 NS pp1ab
n = 7 NS pp1abb
n = 7 SS GP b
n = 7 NS pp1a/NS pp1ab/SS GPb
n = 6
500
LightCycler
28.69 ± 0,46
28.16 ± 0.04
29.57 ± 0.87
29.56 ± 0.18
29.47 ± 0.53
28.53 ± 0.26

SDS7700
n.d.
27.29 ± 0.09
29.63 ± 0.94
30.92 ± 0.09
26.98 ± 0.65
26.11 ± 0.40
50
LightCycler
32.68 ± 0.26
45.00
33.85 ± 0.60
32.83 ± 0.41
32.83 ± 0.41
32.48 ± 0.69

SDS7700
n.d.
31.62 ± 0.45
33.82 ± 0.23
34.73 ± 1.04
30.33 ± 0.26
29.29 ± 0.67
10
LightCycler
34.42 ± 0.20
45.00
36.72 ± 0.42
35.99 ± 0.55
35.33 ± 0.48
34.50 ± 0.25

SDS7700
n.d.
38.37 ± 4.02
35.69 ± 0.35
36.94 ± 0.10
32.93 ± 0.81
32.01 ± 0.44
5
LightCycler
35.66 ± 0.40
45.00
45.00
37.61 ± 0.55
37.12 ± 0.35
37.20 ± 0.30
SDS7700 n.d. 41.15 ± 2.17 37.31 ± 0.77 37.64 ± 0.67 34.55 ± 0.50 34.09 ± 0.24

aCT, threshold cycle; SARS-CoV, severe acute respiratory syndrome–associated coronavirus; RT-PCR, reverse transcription–polymerase chain reaction; GE, genome equivalents/assay; pp1ab, nonstructural polyprotein 1ab gene; n.d., not determined.
bSee Table 1.

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