Volume 10, Number 7—July 2004
Dispatch
SARS Coronavirus Detection
Andreas Nitsche*
, Brunhilde Schweiger*, Heinz Ellerbrok*, Matthias Niedrig*, and Georg Pauli*
, Brunhilde Schweiger*, Heinz Ellerbrok*, Matthias Niedrig*, and Georg Pauli*Table 2
CT values and standard deviation of serial dilutions of SARS-CoV RNA subjected to different real-time RT-PCR assaysa
| GE | Real-time RT-PCR systems | Artus kit n = 4 | Drosten et al. (5) n = 6 | NS pp1ab n = 7 | NS pp1abb n = 7 | SS GP b n = 7 | NS pp1a/NS pp1ab/SS GPb n = 6 | |
|---|---|---|---|---|---|---|---|---|
| 500 |
LightCycler |
28.69 ± 0,46 |
28.16 ± 0.04 |
29.57 ± 0.87 |
29.56 ± 0.18 |
29.47 ± 0.53 |
28.53 ± 0.26 |
|
| SDS7700 |
n.d. |
27.29 ± 0.09 |
29.63 ± 0.94 |
30.92 ± 0.09 |
26.98 ± 0.65 |
26.11 ± 0.40 |
||
| 50 |
LightCycler |
32.68 ± 0.26 |
45.00 |
33.85 ± 0.60 |
32.83 ± 0.41 |
32.83 ± 0.41 |
32.48 ± 0.69 |
|
| SDS7700 |
n.d. |
31.62 ± 0.45 |
33.82 ± 0.23 |
34.73 ± 1.04 |
30.33 ± 0.26 |
29.29 ± 0.67 |
||
| 10 |
LightCycler |
34.42 ± 0.20 |
45.00 |
36.72 ± 0.42 |
35.99 ± 0.55 |
35.33 ± 0.48 |
34.50 ± 0.25 |
|
| SDS7700 |
n.d. |
38.37 ± 4.02 |
35.69 ± 0.35 |
36.94 ± 0.10 |
32.93 ± 0.81 |
32.01 ± 0.44 |
||
| 5 |
LightCycler |
35.66 ± 0.40 |
45.00 |
45.00 |
37.61 ± 0.55 |
37.12 ± 0.35 |
37.20 ± 0.30 |
|
| SDS7700 | n.d. | 41.15 ± 2.17 | 37.31 ± 0.77 | 37.64 ± 0.67 | 34.55 ± 0.50 | 34.09 ± 0.24 | ||
aCT, threshold cycle; SARS-CoV, severe acute respiratory syndrome–associated coronavirus; RT-PCR, reverse transcription–polymerase chain reaction; GE, genome equivalents/assay; pp1ab, nonstructural polyprotein 1ab gene; n.d., not determined.
bSee Table 1.
