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Volume 10, Number 8—August 2004

Research

Serotype III Streptococcus agalactiae from Bovine Milk and Human Neonatal Infections1

John F. Bohnsack*Comments to Author , April A. Whiting*, Gabriela Martinez†, Nicola Jones‡, Elisabeth E. Adderson§, Shauna Detrick*, Anne J. Blaschke-Bonkowsky*, Naiel Bisharat‡, and Marcelo Gottschalk†
Author affiliations: *University of Utah Health Sciences Center, Salt Lake City, Utah, USA; †Faculté de Médicine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada; ‡John Radcliffe Hospital, Oxford, United Kingdom; §St. Jude Children’s Research Hospital, Memphis, Tennessee, USA

Main Article

Table 5

Inserted sequences and sequence types of infB H allele strainsa




Size of PCR poroduct from inserted sequence sites (bp)


RAPD Isolate AW-10 hylB scpb-lmb ST
II
RF-96-2834
3,000b
700c
650d
61

ASS-97-0701
3,000
700
650
61

AL-96-1653
1,700e
700
650
61

SF-96-6312
3,400f
700
650
61g

SF-96-4396
1,700
700
650
91

NI-96-2521
3,000
700
650
105
IV-B
ASS-96-659
3,000
700
No product
61g

SH-96-5461
1,700
700
No product
61
Ungrouped
AL-96-2049
3,000
700
650
61
NI-96-3329 3,000 700 650 61

aRAPD, randomly amplified polymorphic DNA; ST, sequence type.
bA 3,000-bp amplicon was sequenced and found to contain an IS3-like insertion sequence interrupting GBSi1.
cA 700-bp product from the hylB site indicates that there is no inserted sequence in the site.
dA 650-bp product from the scpb-lmb site indicates that there is no inserted sequence in the site.
eA 1,700-bp product was amplified and found to contain an intact copy of GBSi1.
fA 3,400-bp amplicon was sequenced and found to contain GBSi1 and an IS1563-like insertion sequence.
gThe glcK (glucose kinase) gene in these two strains contains a mobile genetic element in the portion of glcK used to determine the ST of GBS strains. These two strains were found to be sequence type 61 (ST-61) after the inserted sequence was removed. The identical inserted sequence in the glcK gene has been found in other ST-61 bovine strains and is described elsewhere (21).

Main Article

1Presented in part at the XVth Lancefield International Symposium on Streptococci and Streptococcal Diseases, October 6–11, 2002, Goa, India.

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