Volume 11, Number 2—February 2005
Dispatch
Diagnostic System for Rapid and Sensitive Differential Detection of Pathogens
Thomas Briese*1, Gustavo Palacios*1, Mark Kokoris†1, Omar Jabado*, Zhiqiang Liu*, Neil Renwick*, Vishal Kapoor*, Inmaculada Casas‡, Francisco Pozo‡, Ron Limberger§, Pilar Perez-Brena‡, Jingyue Ju*, and W. Ian Lipkin*
Table 2
Multiplex pathogen detection by Mass Tag polymerase chain reaction using Masscode-labeled primers in a 30-plex assay with clinical specimens with previously identified pathogens*
| Pathogen | No. positive/no. tested† |
|---|---|
| RSV A | 2/2 |
| RSV B | 3/3 |
| HPIV-1 | 1/1 |
| HPIV-3 | 2/2 |
| HPIV-4 | 2/2 |
| CoV-SARS | 4/4 |
| Metapneumovirus | 2/3 |
| Influenza B | 1/3 |
| Influenza A | 2/6 |
| Adenovirus | 2/2 |
| Enterovirus | 2/2 |
*RSV, respiratory syncytial virus; HPIV, human parainfluenza virus; CoV, coronavirus; SARS, severe acute respiratory syndrome.
†No. positive and consistent with previous diagnosis/number tested (with respective previous diagnosis).
1These authors contributed equally to this study.
