Volume 11, Number 2—February 2005
Dispatch
Diagnostic System for Rapid and Sensitive Differential Detection of Pathogens
Thomas Briese*1, Gustavo Palacios*1, Mark Kokoris†1, Omar Jabado*, Zhiqiang Liu*, Neil Renwick*, Vishal Kapoor*, Inmaculada Casas‡, Francisco Pozo‡, Ron Limberger§, Pilar Perez-Brena‡, Jingyue Ju*, and W. Ian Lipkin*
Table A1
Respiratory panel Mass Tag primers.
| Target – Masscode FWD/REV | Name FWD | Sequence | Name REV | Sequence | Ref. |
|---|---|---|---|---|---|
| FLUAV-M 618/690 | AM-U151 | CATGGAATGGCTAAAGACAAGACC | AM-L397 | AAGTGCACCAGCAGAATAACTGAG | (8) |
| FLUAV-N1 499/439 | NA1-U1078 | ATGGTAATGGTGTTTGGATAGGAAG | NA1-L1352 | AATGCTGCTCCCACTAGTCCAG | (8) |
| FLUAV-N2 658/730 | NA2-U560 | AAGCATGGCTGCATGTTTGTG | NA2-L858 | ACCAGGATATCGAGGATAACAGGA | (8) |
| FLUAV-HA1 650/590 | HA1-U583 | GGTGTTCATCACCCGTCTAACAT | HA1-L895 | GTGTTTGACACTTCGCGTCACAT | (8) |
| FLUAV-HA2 662/539 | H2A208U27 | GCTATGCAAACTAAACGGAATYCCTCC | H2A559L26 | TATTGTTGTACGATCCTTTGGCAACC | |
| FLUAV-HA3-1 586/475 | HA3-U115 | GCTACTGAGCTGGTTCAGAGTTC | HA3-L375 | GAAGTCTTCATTGATAAACTCCAG | (8) |
| FLUAV-HA5 646/395 | HA5hum-u71 | TTACTGTTACACATGCCCAAGACA | HA5hum-L147 | AGGYTTCACTCCATTTAGATCGCA | |
| FLUBV 698/598 |
BHA-U188 |
AGACCAGAGGGAAACTATGCCC |
BHA-L347 |
CTGTCGTGCATTATAGGAAAGCAC |
(8) |
| HCoV-SARS 527/666 | CIID-28891F | AAGCCTCGCCAAAAACGTAC | CIID-29100R | AAGTCAGCCATGTTCCCGAA | |
| HCoV-229E 670/558 | Taq-Co22-418F | GGCGCAAGAATTCAGAACCA | Taq-Co22-636R | TAAGAGCCGCAGCAACTGC | |
| HCoV-OC43 686/548 |
Taq-Co43-270F |
TGTGCCTATTGCACCAGGAGT |
Taq-Co43-508R |
CCCGATCGACAATGTCAGC |
|
| RSV A 467/455 | RSA-U1137 | AGATCAACTTCTGTCATCCAGCAA | RSV-L1192 | GCACATCATAATTAGGAGTATCAAT | (9) |
| RSV B 483/479 |
RSB-U1248 |
AAGATGCAAATCATAAATTCACAGGA |
RSV-1318 |
TGATATCCAGCATCTTTAAGTATCTTTATAGTG |
(9) |
| HMPV-1 718/654 | MPV01.2 | AACCGTGTACTAAGTGATGCACTC | MPV02.2 | CATTGTTTGACCGGCCCCATAA | (10) |
| HMPV-2 718/654 |
MV-Can-U918 |
AAGTCCAAAGGCAGGRCTGTTATC |
MV-Can-L992 |
CCTGAAGCATTRCCAAGAACAACAC |
|
| HPIV-1 566/357 | HPIV1-U82 | TACTTTTGACACATTTAGTTCCAGGAG | HPIV1-L167 | CGGTACTTCTTTGACCAGGTATAATTG | |
| HPIV-2 463/590 | HPIV2-U908 | GGACTTGGAACAAGATGGCCT | HPIV2-L984 | AGCATGAGAGCYTTTAATTTCTGGA | |
| HPIV-3 423/539 | HPIV3-U590 | GCTTTCAGACAAGATGGAACAGTG | HPIV3-L668 | GCATKATTGACCCAATCTGATCC | |
| HPIV 4A 622/606 | HPIV4A-U191 | AACAGAAGGAAATGATGGTGGAAC | HPIV4A-L269 | TGCTGTGGATGTATGGGCAG | |
| HPIV 4B 622/606 |
HPIV4B-U194 |
AGAAGAAAACAACGATGAGACAAGG |
HPIV4B-L306 |
GTTTCCCTGGTTCACTCTCTTCA |
|
| Measles 578/562 | MEA-U1103 | CAAGCATCATGATYGCCATTCCTGG | MEA-L1183 | CCTGAATCYCTGCCTATGATGGGTTT | |
| Adenovirus 503/630 | ADV2HEX2F | CCCMTTYAACCACCACCG | ADVHEX1R | ACATCCTTBCKGAAGTTCCA | (11) |
| Enterovirus 702/495 | 5UTR-U447 | TCCTCCGGCCCCTGAATGCGGCTAATCC | 5UTR-L541 | GAAACACGGWCACCCAAAGTASTCG | |
| HHV-1 443/706 | HSV-U27 | CCCGGATGCGGTCCAGACGATTAT | HSV-L121 | CCCGCGGAGGTTGTACAAAAAGCT | |
| HHV-3 515/471 | VZV-U138 | ACGTGGATCGTCGGATCAGTTGT | VZV-L196 | TCGCTATGTGCTAAAACACGCGG | |
| Legionella pneumophila 678/582 | LegPneu-U149 | GCATWGATGTTARTCCGGAAGCA | LegPneu-L223 | CGGTTAAAGCCAATTGAGCG | |
| Chlamydia pneumoniae 519/383 | CLPM1 | CATGGTGTCATTCGCCAAGT | CLPM2 | CGTGTCGTCCAGCCATTTTA | (12) |
| Streptococcus pneumonia 714/694 | SPPLY-U532 | AGCGATAGCTTTCTCCAAGTGG | SPPLY-L606 | CTTAGCCAACAAATCGTTTACCG | |
| Haemophilus influenzae 734/726 | HINF-U82 | AAGCTCCTTGMATTTTTTGTATTAGAA | Hinf-L158 | GCTGAATTGGCTTRGATACCGAG | |
| Mycoplasma pneumoniae 602/614 | MTPM1 | CCAACCAAACAACAACGTTCA | MTPM2 | ACCTTGACTGGAGGCCGTTA | (12) |
1These authors contributed equally to this study.
