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Volume 11, Number 3—March 2005

Dispatch

Concomitant Tickborne Encephalitis and Human Granulocytic Ehrlichiosis

Stanka Lotric-Furlan*Comments to Author , Miroslav Petrovec†, Tatjana Avsic-Zupanc†, and Franc Strle*
Author affiliations: *University Medical Centre Ljubljana, Slovenia; †Medical Faculty of Ljubljana, Ljubljana, Slovenia

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Table 2

Results of PCR analysis and serum antibody titers to different tickborne pathogens tested at different times after the onset of illness*

Days after onset of illness PCR† 16S RNA gene ELISA IgM/IgG‡ TBEV IFA IgG A. phagocytophilum IFA IgM/IgG E. chaffeensis
Anaplasma phagocytophilum Ehrlichia chaffeensis
12 Pos Neg 0.614/129 Neg Neg/neg
14 Neg Neg 1.369/251 512 Neg/neg
20 1.187/382 2,048 Neg/neg
45 0.419/220 512 Neg/neg
144 0.400/179 512 Neg/neg

*PCR, polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay; IFA, indirect immunofluorescence assay; Ig, immunoglobulin; Neg, negative; Pos, positive; TBEV, tickborne encephalitis virus. †Primers Ehr521 and Ehr790 specific to A. phagocytophilum and primers HE1 and HE3 specific to E. chaffeensis. ‡Cut-off value for TBEV IgM is 0.272–0.372 and for IgG is 7.5 U/mL, according to the manufacturer's procedure

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