Volume 12, Number 2—February 2006
Dispatch
Sequencing and Staphylococci Identification
Alexander Mellmann*
, Karsten Becker*, Christof von Eiff*, Ursula Keckevoet*, Peter Schumann†, and Dag Harmsen*
, Karsten Becker*, Christof von Eiff*, Ursula Keckevoet*, Peter Schumann†, and Dag Harmsen*Table 1
Primers used for amplification and partial sequencing of the partial staphylococcal RNA polymerase B (rpoB) gene
| Primer | Application | Primer sequence (5´→3´) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|
| Staph rpoB 1418f* | Amplification and sequencing | CAA TTC ATG GAC CAA GC | 52 | Modified from 7 |
| Staph rpoB 3554r | Amplification | CCG TCC CAA GTC ATG AAA C | 52 | 7 |
| Staph rpoB 1975r* | Sequencing | GCI ACI TGI TCC ATA CCT GT | 52 | Modified from 7 |
| Staph rpoB 1876r*† | Sequencing | GAG TCA TCI TTY TCT AAG AAT GG | 52 | This study |
*Primers are numbered from the 3´ end of the primer on the forward strand of Staphylococcus aureus (GenBank accession no. X64172).
†Primer was used for sequencing when primer Staph rpoB 1975r did not work.
