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Volume 12, Number 2—February 2006


Sequencing and Staphylococci Identification

Alexander Mellmann*Comments to Author , Karsten Becker*, Christof von Eiff*, Ursula Keckevoet*, Peter Schumann†, and Dag Harmsen*
Author affiliations: *University Hospital Münster, Münster, Germany; †Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany

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Table 1

Primers used for amplification and partial sequencing of the partial staphylococcal RNA polymerase B (rpoB) gene

Primer Application Primer sequence (5´→3´) Annealing temperature (°C) Reference
Staph rpoB 1418f* Amplification and sequencing CAA TTC ATG GAC CAA GC 52 Modified from 7
Staph rpoB 3554r Amplification CCG TCC CAA GTC ATG AAA C 52 7
Staph rpoB 1975r* Sequencing GCI ACI TGI TCC ATA CCT GT 52 Modified from 7
Staph rpoB 1876r*† Sequencing GAG TCA TCI TTY TCT AAG AAT GG 52 This study

*Primers are numbered from the 3´ end of the primer on the forward strand of Staphylococcus aureus (GenBank accession no. X64172).
†Primer was used for sequencing when primer Staph rpoB 1975r did not work.

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