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Volume 12, Number 3—March 2006

Dispatch

Aquariums as Reservoirs for Multidrug-resistant Salmonella Paratyphi B

Renee S. Levings*†, Diane Lightfoot‡, Ruth M. Hall§, and Steven P. Djordjevic*Comments to Author 
Author affiliations: *Elizabeth Macarthur Agricultural Institute, Camden, New South Wales, Australia; †University of Wollongong, Wollongong, New South Wales, Australia; ‡University of Melbourne, Melbourne, Victoria, Australia; §University of Sydney, Sydney, New South Wales, Australia

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Figure 2

Pulsed-field gel electrophoresis (PFGE) and IS200 profiles of Salmonella enterica serovar Paratyphi B dT+ isolates positive for Salmonella genomic island 1. A) PFGE profiles. Xba I–digested whole-cell DNA was separated by PFGE as previously described (12). Molecular mass markers (lane M) are low-range PFGE markers (New England BioLabs, Beverly, MA, USA) composed of concatamers of bacteriophage lambda DNA. The band absent in lane 147 was present in other runs. B) IS200 profiles. PstI digests of w

Figure 2. Pulsed-field gel electrophoresis (PFGE) and IS200 profiles of Salmonella enterica serovar Paratyphi B dT+ isolates positive for Salmonella genomic island 1. A) PFGE profiles. Xba I–digested whole-cell DNA was separated by PFGE as previously described (12). Molecular mass markers (lane M) are low-range PFGE markers (New England BioLabs, Beverly, MA, USA) composed of concatamers of bacteriophage lambda DNA. The band absent in lane 147 was present in other runs. B) IS200 profiles. PstI digests of whole-cell DNA were separated and hybridized with an IS200 digoxigenin (DIG)–labeled probe. Molecular mass markers (lane M) are DIG-labeled bacteriophage lambda DNA digested with HindIII (Roche Diagnostics, Castle Hill, New South Wales, Australia). Primers and polymerase chain reaction conditions used to generate the IS200 probe have been previously described (6).

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