Volume 12, Number 4—April 2006
Research
Lineage 1 and 2 Strains of Encephalitic West Nile Virus, Central Europe
Tamás Bakonyi*†, Éva Ivanics‡, Károly Erdélyi‡, Krisztina Ursu‡, Emőke Ferenczi§, Herbert Weissenböck*, and Norbert Nowotny*¶
Table A2
Oligonucleotide primers used for sequencing of West Nile virus (WNV) strain goshawk-Hungary/04
| Name* | Sequence 5´ to 3´ | Product length (bp) |
|---|---|---|
| WNVII 1f | AGTAGTTCGCCTGTGTGAGC | 200 |
| WNVII 200r | AGCATAGCCCTCTTCAGTCC | |
| WNVII 81f | TGGCACGAAGATCTCGATGT | 874 |
| WNVII 954r | TGCCACCAGGAGCAATAGAA | |
| WNVII 870f | CCTCGTTGCAGCTGTCATTG | 761 |
| WNVII 1630r | TCCATGGCAGGTTCAGATCC | |
| WNVII 1584f | CTTCCTGGTTCACCGAGAAT | 908 |
| WNVII 2491r | CTTGCCTGCCAATGTCAATG | |
| Usu 2328f | CTCTTTGGRGGRATGTCHTG | 818 |
| Usu 3145r | GTGCADGATTTKACYTCTCC | |
| WNVII 3027f | CAACATGGCTGTGCATAGTG | 673 |
| WNVII 3699r | GCCGACGAGAATGACATATC | |
| Usu 3606f | AAGAGGTGGACGGCCARRHT | 1,152 |
| Usu 4759r | GTGTGCCAYAGYGTGTGGAA | |
| WNVII 3861f | GGCTTACTATGACGCCAAGA | 594 |
| WNVII 4454r | CCATCATCATCCAGCCTAAC | |
| Usu 4251f | ATGTTYGCCATYGTWGGDGG | 1,142 |
| Usu 5392r | TGGCACATSACATCMACKAT | |
| WNVII 5294f | TTGCTGCTGAGATGTCTGAG | 324 |
| WNVII 5617r | TGTCCGAGATAGGAGCATTG | |
| Usu 5454f | ATGGATGAAGCYCATTTCAC | 337 |
| Usu 5790r | GGTAYTCYGTMTCATAGGAC | |
| WNVII 5629f | GCCTGGAACACTGGATATGA | 616 |
| WNVII 6245r | TAAGCGAGCCAGACTGGTAA | |
| WNVII 6127f | TATCAGCCTGAGCGCGAGAA | 932 |
| WNVII 7058r | ACGGCTGTCGTTACGGCATA | |
| WNVII 6935f | ATGACATTGGCAGCCTGTTG | 672 |
| WNVII 7606r | ATCCTCCTCGCATGATGTGA | |
| WNVII 7505f | GAGAGGCTGGAATTCTGACT | 653 |
| WNVII 8158r | GTTCTTCTACCTCGGCACTT | |
| WNV 8078f | CATCAGAAGCGAGCGACACA | 744 |
| WNV 8821r | ACAGCCAGTTCGTGGTCTCA | |
| WNV 8723f | TCGGTCAACAACGAGTGTTC | 739 |
| WNV 9461r | GAGATGACGTCCATCACAGT | |
| WNV 9368f | TAGCGCGGTCCATCATCGAG | 733 |
| WNV 10100r | CCAACGTTCGACCGGAGTCT | |
| WNVII 10023f | CTGTTCCGCTGTGCCTGTCA | 519 |
| WNVII 10541r | CGTGGATCACCTCGCAGCTT | |
| WNV 9031f | TACAACATGATGGGVAARAGAGAGA | 1,061 |
| WNV 10091r | AGCATGTCTTCYGTBGTCATCCAYT | |
| WNV 10460f | GCCACCGGAAGTTGAGTAGA | 430 |
| WNV 10889r | GCTGGTTGTGCAGAGCAGAA | |
| Usu 10596f | GWAAGCCTCYCAGAACCGTCTCGGAAG | 419 |
| Usu 11014r | AGATCCTGTGKTCTWSYYCMCCAYCAG |
*Numbers refer to nucleic acid positions at the 5´-end, according to the complete genomic sequence of the closely related lineage 2 WNV strain B956 (NC_001563). Universal, Japanese encephalitis virus–group specific primers are indicated in italics (28). f, forward (genomic) primer; r, reverse (complementary) primer. Usu, Usutu.
