Volume 12, Number 5—May 2006
Dispatch
Enterobacter cloacae Outbreak and Emergence of Quinolone Resistance Gene in Dutch Hospital
Armand Paauw*
, Ad C. Fluit*, Jan Verhoef*, and Maurine A. Leverstein-van Hall*
, Ad C. Fluit*, Jan Verhoef*, and Maurine A. Leverstein-van Hall*Table
Oligonucleotides used for polymerase chain reaction amplification and sequencing
| Target | Primer | 5´–3´ sequences | GenBank accession no. | Nucleotide positions | Annealing temperature (°C) | Amplicon size (bp) | Source |
|---|---|---|---|---|---|---|---|
| QnrA | qnrAR | AGG AAG CGC CGC TGA GAT TG | AY070235 | 762–743 | 56 | 281 | This study |
| qnrAF | CTA TGC CGA TCT GCG CGA TG | AY070235 | 482–501 | This study | |||
| aadB-3´CS | aadB | TGG AGG AGT TGG ACT AT | AY173047 | 251–267 | 55 | 432 | This study |
| 3´CS | AAG CAG ACT TGA CCT GA | M73819 | 1342–1326 | (15) | |||
| blaCTX-M-: most | ctx-m-uni-F | CGA TGT GCA GTA CCA GTA A | U95364 | 214–232 | 50 | 538 | This study |
| ctx-m-uni-R | ATA TCG TTG GTG GTG CC | U95364 | 751–735 | This study | |||
| blaCTX-M-: 2,4,5,6,7,20,Toho-1 | ctx-m-2F | ATG ATG ACT CAG AGC ATT CG | X92507 | 6–25 | 58 | 884 | (16) |
| ctx-m-2R | TTA TTG CAT CAG AAA CCG TG | X92507 | 889–870 | (16) | |||
| blaCTX-M-: 3,10,11,12,15,22,25 | ctx-m-10-1F | ATG GTT AAA AAA TCA CTG CG | X92506 | 63–82 | 60 | 872 | This study |
| ctx-m-10-4R | AAA CCG TTG GTG ACG AT | X92506 | 934–918 | This study | |||
| blaCTX-M-: 9,13,14,15,16,17,18,19,24,Toho-2 and –3 | ctx-m-9F | AGA CGA GTG CGG TGC AGC AA | AJ416345 | 217–236 | 67 | 773 | This study |
| ctx-m-9R | GAT TCT CGC CGC TGA AGC CA | AJ416345 | 989–970 | This study | |||
| Sequence blaCTX-M-9 group | ctx-m-9-1F | TGG TGA CAA AGA GAG TGC AAC G | AJ416345 | 133–154 | This study | ||
| ctx-m-9-MF | GGA GGC GTG ACG GCT TTT | AJ416345 | 576–593 | This study | |||
| ctx-m-9-MR | AAA AGC CGT CAC GCC TCC | AJ416345 | 593–576 | This study | |||
| ctx-m-9-4R | TCA CAG CCC TTC GGC GAT | AJ416345 | 1007–990 | This study |
