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Volume 12, Number 6—June 2006

Research

Norwalk Virus–specific Binding to Oyster Digestive Tissues

Françoise S. Le Guyader*Comments to Author , Fabienne Loisy*, Robert L. Atmar†, Anne M. Hutson†, Mary K. Estes†, Nathalie Ruvoën-Clouet‡§¶, Monique Pommepuy*, and Jacques Le Pendu§¶
Author affiliations: *Institut Français de Recherche pour l'Exploitation de la Mer, Nantes, France; †Baylor College of Medicine, Houston, Texas, USA; ‡Ecole Nationale Vétérinaire, Nantes, France; §Institut National de la Santé et de la Recherche Médicale, Nantes, France; ¶Université de Nantes, Nantes, France

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Table 1

Inhibition of binding of Norwalk viruslike particles (VLPs) to oyster digestive tissues by saliva

Saliva No. samples tested VLP binding*
A 14
O 18 +
B 4 +++
Nonsecretor 5 +++
Control 5 +++

*After incubation with saliva, no (–), weak (+), or strong (+++) VLP binding to oyster digestive tissues was observed. For controls, VLPs were applied to shellfish tissues in phosphate-buffered saline/1% bovine serum albumin instead of a saliva sample diluted in the same buffer.

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