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Volume 13, Number 1—January 2007

Dispatch

Reemergence of Endemic Chikungunya, Malaysia

Sazaly AbuBakar*Comments to Author , I-Ching Sam*, Pooi-Fong Wong*, Poh-Sim Hooi*, Nuruliza Roslan*, and NorAziyah MatRahim
Author affiliations: *University of Malaya, Kuala Lumpur, Malaysia;

Main Article

Table

Identification of virus by PCR amplification and serologic analysis*

Patient
Chikungunya
Dengue fever
Age (y)SexPCR†
Serology
CulturePCR‡Serology
E1E2nsP1IgMIgGIgM
6M+++
34M+++
40M++++#
26F++++**ND
62M+++−††ND
(day 5 after onset)
++NDNDND
(day 15 after onset)

*IgM, immunoglobulin M; IgG, immunoglobulin G; +, positive; −, negative; ND, not determined.
†PCR amplifications were performed for detection of envelope glycoprotein E1 (E1), glycoprotein E2 (E2), and nonstructural protein 1 (nsP1) genes of chikungunya virus.
‡Multiplex PCR amplifications were performed for detection of dengue virus type 1−4.
§Isolate MY/0306/BP37348.
¶Isolate MY/0306/BP37350.
#Isolate MY/0306/BP37352.
**Isolate MY/0406/BP37437.
††Isolate MY/0306/BP34198.

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