Volume 13, Number 1—January 2007
Research
Prevalence of G2P[4] and G12P[6] Rotavirus, Bangladesh
Mustafizur Rahman*†
, Rasheda Sultana*, Giasuddin Ahmed*, Sharifun Nahar*, Zahid M. Hassan*, Farjana Saiada*, Goutam Podder*, Abu S. G. Faruque*, A. K. Siddique*, David A. Sack*, Jelle Matthijnssens†, Marc Van Ranst†, and Tasnim Azim*
, Rasheda Sultana*, Giasuddin Ahmed*, Sharifun Nahar*, Zahid M. Hassan*, Farjana Saiada*, Goutam Podder*, Abu S. G. Faruque*, A. K. Siddique*, David A. Sack*, Jelle Matthijnssens†, Marc Van Ranst†, and Tasnim Azim*Table 1
Oligonucleotide primers used in the study for PCR amplification
| Primer | Type | Position (nt) | Strand | Sequence (5′–3′) | Reference |
|---|---|---|---|---|---|
| Beg9 | VP7 | 1–28 | Plus | GGCTTTAAAAGAGAGAATTTCCGTCTGG | (13) |
| End9 | VP7 | 1062–1036 | Minus | GGTCACATCATACAATTCTAATCTAAG | (13) |
| RVG9 | VP7 | 1062–1044 | Minus | GGTCACATCATACAATTCT | (13) |
| Con2 | VP4 | 868–887 | Minus | ATTTCGGACCATTTATAACC | (11) |
| Con3 | VP4 | 11–32 | Plus | TGGCTTCGCCATTTTATAGACA | (11) |
| MR-G1 | G1 | 314–335 | Plus | CAAGTACTCAAATCAGTGATGG | Present study |
| MR-G2 | G2 | 436–459 | Plus | CTATGAATCCACAACTGTATTGTG | Present study |
| aET3 | G3 | 689–709 | Plus | CGTTTGAAGAAGTTGCAACAG | (13) |
| MR-G4 | G4 | 480–499 | Plus | GCTTCTGGTGAAGAGTTG | Present study |
| aAT8 | G8 | 178–198 | Plus | GTCACACCATTTGTAAATTCG | (13) |
| MR-G9 | G9 | 757–776 | Plus | GAACCATAAACTTGATGTG | Present study |
| MR-P8 | P[8] | 314–335 | Minus | TCTACTGGATCGACGTGC | Present study |
| MR-P4 | P[4] | 474–494 | Minus | CTATTATTAGAGGTTAAAGTC | Present study |
| 3T-1 | P[6] | 259–278 | Minus | TGTTGATTAGTTGGATTCAA | (11) |
| 4T-1 | P[9] | 385–402 | Minus | TGAGACATGCAATTGGAC | (11) |
| ND2 | P[11] | 116–133 | Minus | AGCGAACTCACCAATCTG | (11) |
