Figure 1. A) Histologic analysis of skin nodules caused by human subcutaneous dirofilariasis. Cross-sections of Dirofilaria repens surrounded by an inflammatory granuloma. Note the uteri with developing embryos (hematoxylin and eosin stain, original magnification 10×). B) Analysis of patient samples by agarose gel electrophoresis. Lane 1, 100-bp DNA molecular mass weight marker; lane 2, negative control; lane 3, positive control; lane 4, patient sample showing banding pattern typical of the positive control and similar to the banding pattern described in the original protocol (7).