Figure 3. Antibody ELISA absorbances at 492 nm of 6 viruslike particles (VLPs) of sapovirus. A) Mc114 GI/1; B) Yokote1 GI/5; C) Syd53 GII/3; D) Syd3 GIV/1; E) NK24 GV/1; F) NoV Osaka659. Wells were coated with ≈100 ng of purified VLPs. Antiserum was used in 2-fold dilutions from 1:500 to 1,024,000 in phosphate-buffered saline, 0.1% Tween 20, 5% skim milk shown as 1–12 along the x-axis. OD, optical density; NoV, Norwalk virus.