Emerging Infectious Disease ISSN: 1080-6059

Genetic Diversity among Clonal Lineages within Escherichia coli O157:H7 Stepwise Evolutionary Model

Peter C.H. Feng*

, Steven R. Monday*, David W. Lacher†, Lesley Allison‡, Anja Siitonen§, Christine Keys*, Marjut Eklund§, Hideki Nagano¶, Helge Karch#, James Keen**, and Thomas S. Whittam†

Author affiliations: *Food and Drug Administration, College Park, Maryland, USA; †Michigan State University, East Lansing, Michigan, USA; ‡Western General Hospital, Edinburgh, Scotland; §National Public Health Institute, Helsinki, Finland; ¶Hokkaido Institute of Public Health, Hokkaido, Japan; #University of Munster, Munster, Germany; **US Department of Agriculture, Clay Center, Nebraska, USA;

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Figure 1

Pulsed-field gel electrophoresis of XbaI-digested DNA from GUD+ O157:H7 strains. Strain designation, source and year of isolation are shown at right. This unweighted pair-group method with arithmetic mean dendrogram was generated in BioNumerics software (Applied Maths, St-Martens-Latem, Belgium) by using Die coefficient with a 1.0% lane optimization and 1.0% band position tolerance. The scale above the dendrogram indicates percent similarity.

Figure 1. Pulsed-field gel electrophoresis of XbaI-digested DNA from GUD+ O157:H7 strains. Strain designation, source and year of isolation are shown at right. This unweighted pair-group method with arithmetic mean dendrogram was generated in BioNumerics software (Applied Maths, St-Martens-Latem, Belgium) by using Die coefficient with a 1.0% lane optimization and 1.0% band position tolerance. The scale above the dendrogram indicates percent similarity.

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