Volume 13, Number 12—December 2007
Dispatch
Enhanced Subtyping Scheme for Salmonella Enteritidis
Jie Zheng*, Christine E. Keys†, Shaohua Zhao‡, Jianghong Meng*, and Eric W. Brown†
Table 1
Pulsed-field gel electrophoresis run conditions for 6 restriction enzymes used to subtype Salmonella Enteritidis*
| Enzyme | Digestion temperature, °C | Enzyme units, per plug | Run time, h† | Initial switch time, s | Final switch time, s |
|---|---|---|---|---|---|
| XbaI | 37 | 50 | 19 | 2.16 | 63.8 |
| BlnI | 37 | 30 | 19 | 2.16 | 63.8 |
| SpeI | 37 | 30 | 20.5 | 5 | 25 |
| SfiI | 50 | 30 | 20.5 | 5 | 25 |
| PacI | 37 | 30 | 20.5 | 0.1 | 15 |
| NotI | 37 | 30 | 20.5 | 0.1 | 15 |
*All digestion times were 2–3 h.
†Digestions were separated in 1% agarose gels (Cambrex, Baltimore, MD, USA) at 6V/cm by using a CHEF-Mapper (Bio-Rad, Hercules, CA, USA).
