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Volume 13, Number 12—December 2007

Dispatch

Enhanced Subtyping Scheme for Salmonella Enteritidis

Jie Zheng*, Christine E. Keys†, Shaohua Zhao‡, Jianghong Meng*, and Eric W. Brown†Comments to Author 
Author affiliations: *University of Maryland, College Park, Maryland, USA; †US Food and Drug Administration, College Park, Maryland, USA; ‡US Food and Drug Administration, Laurel, Maryland, USA;

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Table 1

Pulsed-field gel electrophoresis run conditions for 6 restriction enzymes used to subtype Salmonella Enteritidis*

Enzyme Digestion temperature, °C Enzyme units, per plug Run time, h† Initial switch time, s Final switch time, s
XbaI 37 50 19 2.16 63.8
BlnI 37 30 19 2.16 63.8
SpeI 37 30 20.5 5 25
SfiI 50 30 20.5 5 25
PacI 37 30 20.5 0.1 15
NotI 37 30 20.5 0.1 15

*All digestion times were 2–3 h.
†Digestions were separated in 1% agarose gels (Cambrex, Baltimore, MD, USA) at 6V/cm by using a CHEF-Mapper (Bio-Rad, Hercules, CA, USA).

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