Volume 13, Number 12—December 2007
Research
Susceptibility of Canada Geese (Branta canadensis) to Highly Pathogenic Avian Influenza Virus (H5N1)
John Pasick*
, Yohannes Berhane*, Carissa Embury-Hyatt*, John Copps*, Helen Kehler*, Katherine Handel*, Shawn Babiuk*, Kathleen Hooper-McGrevy*, Yan Li†, Quynh Mai Le‡, and Song Lien Phuong§
, Yohannes Berhane*, Carissa Embury-Hyatt*, John Copps*, Helen Kehler*, Katherine Handel*, Shawn Babiuk*, Kathleen Hooper-McGrevy*, Yan Li†, Quynh Mai Le‡, and Song Lien Phuong§Table 1
NP and H5 antibody levels in juvenile and adult Canada geese*
| Animal ID | 0 dpi cELISA (NP % inhibition) | 0 dpi H5 HI assay† | 14 dpi (H5N2) cELISA (NP % inhibition) | 21 dpi (H5N2) H5 HI assay† | 20–21 dpi (H5N1) cELISA (NP) | 20–21 dpi (H5N1) H5 HI assay† |
|---|---|---|---|---|---|---|
| Juveniles | ||||||
| 852S/27R | Neg (20) | <8 | Pos (66) | 16 | Euthanized or died‡ | |
| 853S/28R | Neg (13) | <8 | Pos (64) | 64 | Pos (46% inhibition) | 8 |
| 856S/31R | Neg (21) | <8 | Pos (57) | 256 | Euthanized | |
| 858S/33R | Neg (22) | <8 | Pos (55) | 128 | Pos (48% inhibition) | 64 |
| 859S/34R | Neg (19) | <8 | Pos (49) | 256 | Euthanized | |
| 851S/26R | Neg (24) | <8 | Euthanized | |||
| 854S/29R | Neg (23) | <8 | Euthanized | |||
| 855S/30R | Neg (22) | <8 | Euthanized | |||
| 860S/35R | Neg (24) | <8 | Euthanized | |||
| 861S/36R | Neg (20) | <8 | ||||
| 857S/32Y | Neg (18) | <8 | ||||
| Adults | ||||||
| 842S/42Y | Pos (93) | <8 | Pos (99) | 512 | Euthanized | |
| 844S/44Y | Neg (23) | <8 | Pos (99) | 64 | Pos (46% inhibition) | <8 |
| 845S/45Y | Pos (58) | <8 | Pos (96) | 8 | Euthanized | |
| 846S/46Y | Pos (76) | <8 | Pos (96) | <8 | Pos (63% inhibition) | <8 |
| 847S/47Y | Pos (74) | <8 | Pos (99) | ND | Euthanized | |
| 840S/40Y | Pos (45) | <8 | Pos (99% inhibition) | >4,096 | ||
| 841S/41Y | Neg (22) | <8 | Euthanized | |||
| 843S/43Y | Pos (78) | <8 | Euthanized | |||
| 848S/48Y | Pos (39) | <8 | Pos (98% inhibition) | 64 | ||
| 849S/49Y | Pos (93) | <8 | Pos (98% inhibition) | 32 | ||
| 850S/50Y | Pos (85) | <8 |
*NP, nucleoprotein; cELISA, competitive ELISA; dpi, days postinfection; Neg, negative (<30% inhibition); Pos, positive (≥30% inhibition); HI, hemagglutinin inhibition; ND, not determined.
†4 hemagglutinin units of A/duck/British Columbia/26–6/2005 (H5N2) used in assay.
‡Euthanized or died before day 20–21 postinoculation with virus (H5N1).
