Volume 13, Number 12—December 2007
Research
Susceptibility of Canada Geese (Branta canadensis) to Highly Pathogenic Avian Influenza Virus (H5N1)
John Pasick*
, Yohannes Berhane*, Carissa Embury-Hyatt*, John Copps*, Helen Kehler*, Katherine Handel*, Shawn Babiuk*, Kathleen Hooper-McGrevy*, Yan Li†, Quynh Mai Le‡, and Song Lien Phuong§
, Yohannes Berhane*, Carissa Embury-Hyatt*, John Copps*, Helen Kehler*, Katherine Handel*, Shawn Babiuk*, Kathleen Hooper-McGrevy*, Yan Li†, Quynh Mai Le‡, and Song Lien Phuong§Table 2
Distribution of influenza virus antigen in tissues of naïve juvenile Canada geese tissues after challenge with influenza virus (H5N1)
| Tissue | Animal 861S/36R dpi 3* | Animal 855S/30R dpi 4* | Animal 851S/26R dpi 5* | Animal 854S/29R dpi 5* | Animal 860S/35R dpi 5* | IHC-positive cell types |
|---|---|---|---|---|---|---|
| Trachea | + | + | – | – | – | Vascular endothelium |
| Lung | ++ | ++ | – | + | – | Vascular endothelium, mononuclear cells |
| Esophagus | ++ | ++ | + | + | – | Epithelium, Vascular smooth muscle, Smooth muscle of muscularis externa, Mucous glands |
| Proventriculus | + | + | – | ++ | – | Epithelium (columnar, glandular), muscularis mucosa, vascular smooth muscle, parasympathetic ganglia |
| Ventriculus | ++ | ++ | – | ++ | – | Epithelium |
| Gut | ++ | +++ | – | ++ | + | Parasympathetic ganglia, mucosal smooth muscle, vascular endothelium |
| Cecal tonsil | ++ | ++ | + | ++ | – | Parasympathetic ganglia |
| Pancreas | ++ | +++ | ++ | ++ | + | Exocrine acinar cells |
| Liver | – | – | – | + | – | Hepatocytes |
| Spleen | ++ | – | + | – | – | Vascular smooth muscle, mononuclear cells |
| Kidney | + | – | – | – | – | Tubular epithelium |
| Muscle | + | + | – | – | – | Vascular smooth muscle |
| Heart | ++ | ++ | ++ | + | + | Myocytes |
| Brain | ++ | +++ | +++ | +++ | +++ | Neurons, glial cells, ependymal cells, choroid plexus epithelium |
| Spinal cord | + | ++ | ++ | ++ | ++ | Ependymal cells, neurons, glial cells, leptomeninges |
| Sciatic nerve | + | – | – | – | – | Vascular endothelium |
| Brachial nerve | – | – | – | – | – | None |
*Numbers of immunohistochemically positive cells: +, few; ++, moderate; +++, numerous; –, virus antigen negative; dpi, days postinfection.
