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Volume 13, Number 12—December 2007

Dispatch

Parachlamydia spp. and Related Chlamydia-like Organisms and Bovine Abortion

Nicole Borel*, Silke Ruhl*, Nicola Casson†, Carmen Kaiser*, Andreas Pospischil*, and Gilbert Greub†Comments to Author 
Author affiliations: *University of Zurich, Zurich, Switzerland; †University of Lausanne, Lausanne, Switzerland;

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Figure

A) Immunohistochemical analysis of a bovine placenta positive by PCR for Parachlamydia acanthamoebae, showing a positive brown-red granular reaction within trophoblastic epithelium. Antigen detection was conducted with a polyclonal antibody against Parachlamydia spp. (3-amino-9-ethylcarbazole/peroxidase method, hematoxylin counterstain). B) Transmission electron micrograph of bovine placenta positive by PCR and immunohistochemical analysis for P. acanthamoebae, showing 7 cocci-shaped bacteria in an inclusion with morphologic features similar to those of Chlamydia-like organisms (12).

Figure. A) Immunohistochemical analysis of a bovine placenta positive by PCR for Parachlamydia acanthamoebae, showing a positive brown-red granular reaction within trophoblastic epithelium. Antigen detection was conducted with a polyclonal antibody against Parachlamydia spp. (3-amino-9-ethylcarbazole/peroxidase method, hematoxylin counterstain). B) Transmission electron micrograph of bovine placenta positive by PCR and immunohistochemical analysis for P. acanthamoebae, showing 7 cocci-shaped bacteria in an inclusion with morphologic features similar to those of Chlamydia-like organisms (12).

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