Figure. A) Immunohistochemical analysis of a bovine placenta positive by PCR for Parachlamydia acanthamoebae, showing a positive brown-red granular reaction within trophoblastic epithelium. Antigen detection was conducted with a polyclonal antibody against Parachlamydia spp. (3-amino-9-ethylcarbazole/peroxidase method, hematoxylin counterstain). B) Transmission electron micrograph of bovine placenta positive by PCR and immunohistochemical analysis for P. acanthamoebae, showing 7 cocci-shaped bacteria in an inclusion with morphologic features similar to those of Chlamydia-like organisms (12).