Figure. Phylogenetic analysis of Canadian WU polyomavirus strains CanWUT9–2003, CanWUT38–2003, CanWUC40–2003 and CanWUT52–2003 (shown in boldface), based on nucleotide sequences of the VP2 region. Multiple nucleotide sequence alignments were performed by using the ClustalW program and a phylogenetic tree was constructed with the MEGA 3.1 software using the neighbor-joining algorithm with Kimura-2 parameters (9). The analysis included WU strains previously identified from Australian and American cohorts (7) i.e., B9, S6, B28, B37, B22, B24, B35, B10, B1, B17, B0, B12, B20, B14, B34, B21, B3, and B2 (GenBank accession nos.: EF444592, EF444593, EF444590, EF444589, EF444588, EF444587, EF444586, EF444584, EF444583, EF444582, EF-444555, EF444557, EF444561, EF444562, EF444572, EF444585, EF444567, and EF444591, respectively).