Figure 2. Genetic context of various insertion element common region (ISCR2) elements. Schematic of various GenBank sequences that harbor the ISCR2 element. Open reading frames (ORFs) are depicted as open boxes with the direction of transcription of the various ORF indicated with arrows. The ORF of the ISCR2 transposase is highlighted, and the limits of the ISCR2 element are shown with vertical lines. The ISCR2 element consists of an ORF of 1491 bp together with ≈120 bp of upstream sequence and ≈240 bp of downstream sequence. The ISCR2 element identified in this study associated with the floR resistance gene is amplified to show primer sites and positions of the putative oriIS and terIS of the ISCR2 element. The bold arrow indicates the direction of rolling circle replication of the ISCR2 element. GenBank accession numbers of the various sequences are indicated. P., Pasteurella; A., Aeromonas; S. enterica, Salmonella enterica; S.maltophilia, Stenotrophomonas maltophila. *This study.
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