Volume 13, Number 5—May 2007
Dispatch
Fatal Disseminated Acanthamoeba lenticulata Acanthamebiasis in a Heart Transplant Patient
Stéphane Barete*
, Alain Combes†, Johan F. de Jonckheere‡, Annick Datry†, Shaïda Varnous†, Valérie Martinez†, Sara García Ptacek*, Eric Caumes†, Frédérique Capron†, Camille Francès*, Claude Gibert†, and Olivier Chosidow*
, Alain Combes†, Johan F. de Jonckheere‡, Annick Datry†, Shaïda Varnous†, Valérie Martinez†, Sara García Ptacek*, Eric Caumes†, Frédérique Capron†, Camille Francès*, Claude Gibert†, and Olivier Chosidow*Table
rDNA sequences of Acanthamoeba isolate (2/533) from the patient, a keratitis isolate (GAK1), 3 environmental T5 subtypes, and 4 other genotypes from persons with nonkeratitis infections
| Genotype (strain) | DF3 sequence (5′→3′)* |
|---|---|
| T5 (2/533) | CAAAACACCGCCGTTAATCCTTTTT---CGGGGGTTAACGGTTGGTGAAT |
| T5 (GAK1) | caaaacaccgccgttaatccttt-----cgggggttaatggttggtgaat |
| T5 (72/2)† | caaaacaccgccgttaatccttt-----cgggggttaatggttggtgaat |
| T5 (PD2S)‡ | caaaacaccgctgttaatccttt-----cgggggttaatagttggtgaat |
| T5 (FLAIV)§ | caaaacaccgccgttaatcctttt-caacgggggttaacggttggtgaat |
| T4 | caaaacacca-Atcggcgcggtcgtccttggcgtcggtccttcacggggccggcgcgagggcggcttagcccggtggcacc |
| T1 | caaaacacca-ccatcaggcagtggggtcgtgcttcgcttttccggcaacggggaagtggaggcggtctcattcccctgatgg |
| T10 | caaaacaccatccatttagcayggtcgttttcaaatattcctttttgcgaaggttgtttgggaacgattcgtcctgatggatc |
| T12 | caaaacacca-ccattaacacgatcgttttttgcaaatatgccacatgcgcaagtgtgtggttgtgtttgaaggaacgatttg |
*Sequences differences are shown in boldface.
†Five isolates at European Molecular Biology Laboratory (EMBL).
‡Eight isolates at EMBL.
§One isolate at EMBL.
