Volume 13, Number 6—June 2007
Research
Minority-Variant pfcrt K76T Mutations and Chloroquine Resistance, Malawi
Jonathan J. Juliano*, Jesse J. Kwiek*, Kathryn Cappell*, Victor Mwapasa†, and Steven R Meshnick*
Table 2
Sensitivity testing of HTA on mixes of genomic DNA at known concentrations*
| Phosphorimager data |
||||
|---|---|---|---|---|
| % Mutant | Average wild-type (%) | SD weight | Average mutant (%) | SD mutant |
| 50 | 48.1 | 2.39 | 51.9 | 2.39 |
| 20 | 77.0 | 2.56 | 23.0 | 2.55 |
| 10 | 88.9 | 2.34 | 11.1 | 2.34 |
| 5 | 94.8 | 0.95 | 5.2 | 0.95 |
| 1 | 98.7 | 0.22 | 1.3 | 0.22 |
| 0.1 | 100 | 0 | ND | ND |
*HTA, heteroduplex tracking assay; ND, none detected.
