Figure 3. Representative immunoblot showing the relative amount of disease-associated prion protein (PrPres) in brain, tonsil, and selected lymph nodes from a single chronic wasting disease (CWD)–affected mule deer. All lanes were loaded with 10-mg equivalents of tissue (original wet weight basis). Lane 1, brain; lane 2, tonsil; lane 3, popliteal lymph node; lane 4, retropharyngeal lymph node (RPLN); lane 5, prescapular lymph node; lane 6, submandibular lymph node; lane 7, mesenteric lymph node. PrPres bands were visualized by using antibody L42 at 0.04 μg/mL and standard enhanced chemiluminescence processing.