Figure. Phylogenetic analysis of Japanese encephalitis virus strains predicted from premembrane gene sequences. Neighbor-joining tree was generated by using MEGA 3.1 software (www.megasoftware.net) and rooted with Murray Valley encephalitis (MVE) virus sequence information. Bootstrap confidence limits for 1,000 replicates are indicated above each branch. Horizontal branch lengths are proportional to genetic distance; vertical branch lengths have no significance. Scale bar indicates no. nucleotide substitutions per site. All sequences from this study are in boldface. Genotypes are indicated on the right. Designations are listed first, followed by country, source, and year of isolation. CSF, cerebrospinal fluid.