Skip directly to local search Skip directly to A to Z list Skip directly to navigation Skip directly to site content Skip directly to page options
CDC Home

Volume 13, Number 9—September 2007

Dispatch

Reduced Sensitivity of Influenza A (H5N1) to Oseltamivir

Jennifer L. McKimm-Breschkin*Comments to Author , Paul W. Selleck†, Tri Bhakti Usman‡, and Michael A. Johnson†
Author affiliations: *CSIRO Molecular and Health Technologies, Parkville, Victoria, Australia; †CSIRO Livestock Industries, Geelong, Victoria, Australia; ‡Disease Investigation Centre Region IV, Yogyakarta, Indonesia;

Main Article

Figure

Sensitivity of clade 1 and clade 2 influenza A (H5N1) viruses to zanamivir, oseltamivir, and 4-amino-Neu5Ac2en in a MUNANA-based enzyme inhibition assay (Sigma, Saint Louis, MO, USA). Viruses were grown in allantoic fluid and irradiated for testing sensitivities of their neuraminidases. Plots are the mean values for inhibition of enzyme activity for each drug concentration of all isolates from that country and year; bars represent standard deviations of values for all isolates from that group. A) Sensitivity to zanamivir; B) sensitivity to oseltamivir; C) sensitivity to 4-amino-Neu5Ac2en.

Figure. Sensitivity of clade 1 and clade 2 influenza A (H5N1) viruses to zanamivir, oseltamivir, and 4-amino-Neu5Ac2en in a MUNANA-based enzyme inhibition assay (Sigma, Saint Louis, MO, USA). Viruses were grown in allantoic fluid and irradiated for testing sensitivities of their neuraminidases. Plots are the mean values for inhibition of enzyme activity for each drug concentration of all isolates from that country and year; bars represent standard deviations of values for all isolates from that group. A) Sensitivity to zanamivir; B) sensitivity to oseltamivir; C) sensitivity to 4-amino-Neu5Ac2en.

Main Article

1Isolates tested were the following: A/Mississippi/3/2001 wt, A/Mississippi/3/2001 H274Y; A/quail/Kelantan/6309/2004, A/chicken/Kelantan/5858/2004, A/quail/Vietnam/007B/2004, A/duck/Vietnam/007A/2004, A/chicken/Vietnam/0024/2004, A/chicken/Vietnam/0023/2004 A/chicken/Vietnam/0018/2004, A/chicken/Vietnam/0015/2004, A/chicken/Vietnam/0010/2004, A/chicken/Vietnam/008/2004; A/chicken/Cambodia/Siem Reap/77B/2004, A/chicken/Cambodia/Siem Reap/76/2004, A/chicken/Cambodia/Takeo/45/2004, A/chicken/Cambodia/Kandal/23/2004, A/chicken/Cambodia/Pong Peay/1B/2004, A/chicken/Cambodia/Pong Peay/1A/2004, A/goose/Cambodia/Kandal/2005, A/chicken/Cambodia/Kandal/3/2005, A/chicken/Cambodia/Kandal/2/2005, A/chicken/Cambodia/Kandal/1/2005, A/chicken/Indonesia/Wates/130/2005, A/chicken/Indonesia/Wates/126/2005, A/chicken/Indonesia/Wates/83/2005, A/chicken/Indonesia/Wates/80/2005, A/chicken/Indonesia/Wates/77/2005, A/chicken/Indonesia/Wates/1/2005.

Top of Page

 

Past Issues

Select a Past Issue:

Art in Science - Selections from Emerging Infectious Diseases
Now available for order



CDC 24/7 – Saving Lives, Protecting People, Saving Money. Learn More About How CDC Works For You…

USA.gov: The U.S. Government's Official Web PortalDepartment of Health and Human Services
Centers for Disease Control and Prevention   1600 Clifton Rd. Atlanta, GA 30333, USA
800-CDC-INFO (800-232-4636) TTY: (888) 232-6348 - Contact CDC–INFO