Volume 14, Number 1—January 2008
THEME ISSUE
International Polar Year
Dispatch
Molecular Epidemiology of Dengue Virus Strains from Finnish Travelers
Eili Huhtamo*
, Nathalie Y. Uzcátegui*, Heli Siikamäki†, Auli Saarinen*, Heli Piiparinen*, Antti Vaheri*, and Olli Vapalahti*‡§
, Nathalie Y. Uzcátegui*, Heli Siikamäki†, Auli Saarinen*, Heli Piiparinen*, Antti Vaheri*, and Olli Vapalahti*‡§Table
Dengue virus isolates from Finnish travelers, 2000–2005*
| Virus serotype | Patient travel history | Year | Isolate/ case no. | Strain designation (GenBank accession no.) | IFA screening of infected cells† |
Isolation serum antibody status |
Patient sex/ age, y | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vero E6 |
C6/36 |
|||||||||||||
| dpi | Pos | dpi | Pos | dpo | IgM | IgG | ||||||||
| DENV-1 | Thailand | 2002 | 3 | F9.D1.02 (EU005250) | 7 | +++ | 7 | ++ | 5 | – | <10 | F/23 | ||
| DENV-1 | Malaysia/ Thailand | 2002 | 4 | F12.D1.02 (EU005249) | – | – | 24 | + | 7 | + | 20 | F/43 | ||
| DENV-1 | Thailand | 2005 | 8 | F31.D1.05 (EU005248) | – | – | 24 | + | 7 | + | <10 | F/56 | ||
| DENV-1 | India | 2005 | 11 | F37.D1.05 (EU005247) | 5 | +++ | 5 | +++ | 3 | NA | NA | M/31 | ||
| DENV-2 | Sri Lanka | 2003 | 6 | F18.D2.03 (EU005252) | – | – | 24 | + | 5 | + | 320 | M/54 | ||
| DENV-2 | Ghana | 2005 | 9 | F32.D2.05 (C-preM, EU005251; E, EU005258) | 7 | +++ | 7 | + | 2 | + | <10 | F/22 | ||
| DENV-3 | Cuba | 2002 | 2 | F7.D3.02 (EU005253) | 7 | + | 24 | + | 6 | + | 20 | M/55 | ||
| DENV-3 | Brazil | 2003 | 5 | F13.D3.03 (EU005254) | – | – | 24 | + | 5 | + | <20 | M/26 | ||
| DENV-3 | Sri Lanka | 2004 | 7 | F24.D3.04 (EU005255) | 4 | +++ | 24 | + | 2 | – | 20 | F/39 | ||
| DENV-4 | Sri Lanka | 2000 | 1 | F2.D4.00 (EU005256) | 10 | +++ | 10 | + | 4 | + | <10 | F/42 | ||
| DENV-4 | Indonesia | 2005 | 10 | F34.D4.05 (EU005257) | – | – | 24 | + | 5 | + | 20 | M/37 | ||
*IFA, immunofluoresence assay; dpi, day postinfection; Pos, proportion of positive cells; dpo, day post-onset; Ig, immunoglobulin; DENV, dengue virus; NA, not available; c-preM, capsid–premembrane; E, envelope.
† +, ++, and +++ indicate the relative amount of positive cells observed in IFA using fluorescence microscope: individual positive cells observed <10% (+), 10–50% of the cells positive (++), 50%–100% of the cells positive (+++). –, not detected.
