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Volume 14, Number 1—January 2008

Dispatch

Protochlamydia naegleriophila as Etiologic Agent of Pneumonia

Nicola Casson*, Rolf Michel†, Karl-Dieter Müller, John David Aubert*, and Gilbert Greub*Comments to Author 
Author affiliations: *University Hospital Center and University of Lausanne, Lausanne, Switzerland; †Central Institute of the Federal Armed Forces Medical Services, Koblenz, Germany; ‡Institut für Medizinische Mikrobiologie der Universität Essen, Essen, Germany;

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Figure 2

A) Intra and inter-run reproducibility of the real-time PCR assessed on duplicate of plasmidic positive controls performed at 10-fold dilutions from 105 to 101 plasmid/μL during 6 successive runs. Standard deviations show the intra-run reproducibility of the real-time PCR. B) Plots of the cycle threshold (Ct) of first and second duplicates, showing intra-run and inter-run variability of the real-time PCR between duplicates of positive control. 95% confidence interval is shown by the dashed lines. C) Bland-Altman graph showing the difference of Ct of both duplicates according to the mean of the Ct of duplicates. The dashed line shows the 95% confidence interval (i.e., limit of agreement).

Figure 2. A) Intra and inter-run reproducibility of the real-time PCR assessed on duplicate of plasmidic positive controls performed at 10-fold dilutions from 105 to 101 plasmid/μL during 6 successive runs. Standard deviations show the intra-run reproducibility of the real-time PCR. B) Plots of the cycle threshold (Ct) of first and second duplicates, showing intra-run and inter-run variability of the real-time PCR between duplicates of positive control. 95% confidence interval is shown by the dashed lines. C) Bland-Altman graph showing the difference of Ct of both duplicates according to the mean of the Ct of duplicates. The dashed line shows the 95% confidence interval (i.e., limit of agreement).

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