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Volume 14, Number 1—January 2008

Dispatch

Protochlamydia naegleriophila as Etiologic Agent of Pneumonia

Nicola Casson*, Rolf Michel†, Karl-Dieter Müller, John David Aubert*, and Gilbert Greub*Comments to Author 
Author affiliations: *University Hospital Center and University of Lausanne, Lausanne, Switzerland; †Central Institute of the Federal Armed Forces Medical Services, Koblenz, Germany; ‡Institut für Medizinische Mikrobiologie der Universität Essen, Essen, Germany;

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Appendix Figure

A) Growth rate of Protochlamydia naegleriophila within Acanthamoeba castellanii assessed using a specific quantitative real-time PCR. Number of DNA copies present in culture are plotted according to time postinfection. Standard errors of the mean of duplicate experiments are shown. B) Indirect immunofluorescence preformed using rabbit anti-KNic antibody directly on the bronchoalveolar lavage showing the presence of few Pr. naegleriophila strain KNic or C) in clusters of this obligate intracellular bacteria. D) Immunofluorescence performed on amebal coculture showing the presence of Pr. naegleriophila. This strain was lost in subsequent passages.

Appendix Figure. A) Growth rate of Protochlamydia naegleriophila within Acanthamoeba castellanii assessed using a specific quantitative real-time PCR. Number of DNA copies present in culture are plotted according to time postinfection. Standard errors of the mean of duplicate experiments are shown. B) Indirect immunofluorescence preformed using rabbit anti-KNic antibody directly on the bronchoalveolar lavage showing the presence of few Pr. naegleriophila strain KNic or C) in clusters of this obligate intracellular bacteria. D) Immunofluorescence performed on amebal coculture showing the presence of Pr. naegleriophila. This strain was lost in subsequent passages.

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