Parachlamydia acanthamoebae Infection and Abortion in Small Ruminants
Silke Ruhl, Genevieve Goy, Nicola Casson, Rudolf Thoma, Andreas Pospischil, Gilbert Greub, and Nicole Borel
Author affiliations: University of Zurich, Zurich, Switzerland (S. Ruhl, A. Pospischil, N. Borel); University of Lausanne, Lausanne, Switzerland (G. Goy, N. Casson, G. Greub); Cantonal Laboratory of Veterinary Bacteriology, Chur, Switzerland (R. Thoma)
Figure. A) Sheep placenta positive by real-time PCR and immunohistochemistry for Parachlamydia spp. and Chlamydiaceae. Chlamydophila abortus was identified by ArrayTube Microarray. Necrotizing placentitis and vasculitis are shown (hematoxylin and eosin stain; magnification ×200). B) Fetal lung of the sheep abortion specimen positive by real-time PCR and immunohistochemical tests for Parachlamydia spp. and Chlamydiaceae; interstitial pneumonia is shown (hematoxylin and eosin stain; magnification ×200). C) Fetal lung that was positive by real-time PCR and immunohistochemical testing for Parachlamydia spp. Positive granular material can be seen within the lung tissue. Antigen detection (immunohistochemistry) was carried out with a polyclonal antibody directed against Parachlamydia spp. 3-amino-9-ethylcarbazole/peroxidase method (hematoxylin counterstain; magnification ×200). D) Double immunohistochemical labeling of the sheep placenta that was positive by real-time PCR and immunohistochemical tests for Chlamydiaceae and Parachlamydia spp. The simultaneous presence of Chlamydiaceae and Parachlamydia spp. granular reaction is shown within necrotic trophoblastic epithelium and neutrophilic exudate (diaminobenzidine/AEC/peroxidase method, hematoxylin counterstain; magnification ×1,000).
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