Emerging Infectious Disease ISSN: 1080-6059

Molecular Detection of Ehrlichia chaffeensis in Amblyomma parvum Ticks, Argentina

Laura Tomassone

, Pablo Nuñez, Ricardo E. Gürtler, Leonardo A. Ceballos, Marcela M. Orozco, Uriel D. Kitron, and Marisa Farber

Author affiliations: Università di Torino, Torino, Italy (L. Tomassone); Instituto de Biotecnología, INTA, Castelar, Argentina (P. Nuñez, M. Farber); Universidad de Buenos Aires, Buenos Aires, Argentina (R.E. Gürtler, L.A. Ceballos, M.M. Orozco); Emory University, Atlanta, Georgia, USA (U.D. Kitron)

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Figure

Agarose gel electrophoresis of PCR products amplified with Ehrlichia chaffeensis (Ec) variable-length PCR target primers. Rc, Rickettsia conorii (negative control). The sources of DNA templates used for amplification are Amblyomma parvum ticks collected from different hosts: A) 1–5 humans; B) 1 dog, 2 foxes, 3–6 cattle, 7–8 goats. Variable amplicon size represents different genotypes that result from differences in the number of tandem repeats in the 5′ end of the variable-length PCR target; PCR products’ sizes range from 500 bp to 600 bp.

Figure. Agarose gel electrophoresis of PCR products amplified with Ehrlichia chaffeensis (Ec) variable-length PCR target primers. Rc, Rickettsia conorii (negative control). The sources of DNA templates used for amplification are Amblyomma parvum ticks collected from different hosts: A) 1–5 humans; B) 1 dog, 2 foxes, 3–6 cattle, 7–8 goats. Variable amplicon size represents different genotypes that result from differences in the number of tandem repeats in the 5′ end of the variable-length PCR target; PCR products’ sizes range from 500 bp to 600 bp.

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