Emerging Infectious Disease ISSN: 1080-6059

Genetic Characterization of Toggenburg Orbivirus, a New Bluetongue Virus, from Goats, Switzerland

Martin A. Hofmann

, Sandra Renzullo, Markus Mader, Valérie Chaignat, Gabriella Worwa, and Barbara Thuer

Author affiliations: Institute of Virology and Immunoprophylaxis, Mittelhaeusern, Switzerland

Figure 1

Nucleotide sequence alignment of target regions of published bluetongue virus (BTV) real-time reverse transcription–PCR primers and probes (in boldface), which were used for detection of Toggenburg orbivirus (TOV). A) Segment 10 (10); B) segment 1, eastern serotype specific (13); C) segment 1, western serotype specific (13); D) segment 1 (14); E), segment 5 (14). Shaded areas indicate primer and probe sequences (in sense orientation), colons indicate sequence identity, arrows indicate orientations of probes and primers, and asterisks indicate mismatches between primers/probes and TOV genome sequence.

Figure 1. Nucleotide sequence alignment of target regions of published bluetongue virus (BTV) real-time reverse transcription–PCR primers and probes (in boldface), which were used for detection of Toggenburg orbivirus (TOV). A) Segment 10 (10); B) segment 1, eastern serotype specific (13); C) segment 1, western serotype specific (13); D) segment 1 (14); E), segment 5 (14). Shaded areas indicate primer and probe sequences (in sense orientation), colons indicate sequence identity, arrows indicate orientations of probes and primers, and asterisks indicate mismatches between primers/probes and TOV genome sequence.

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