Volume 14, Number 12—December 2008
Research
Genetic Characterization of Toggenburg Orbivirus, a New Bluetongue Virus, from Goats, Switzerland
Martin A. Hofmann
, Sandra Renzullo, Markus Mader, Valérie Chaignat, Gabriella Worwa, and Barbara Thuer
, Sandra Renzullo, Markus Mader, Valérie Chaignat, Gabriella Worwa, and Barbara ThuerTable 1
Terminal primers used to amplify complete Toggenburg orbivirus RNA segments
| RNA segment | Name* |
Sequence (5′ → 3′) |
|---|---|---|
| Forward primer | ||
| 2 | BTV4&10_S2_-21_F | GGGTTAAAAGAGTGTTCYAC |
| 5 | BTV_S5_F | GTTAAAAAAGTTCTCTAGTTGGCA |
| 6 | BTV4&10&11_S6_-30_F | GTTAAAAAGTRTTCTCCTACTC |
| 7 | BTV_S7_-17_F | GTTAAAAATCTATAGAGATGGAC |
| 8 | BTV_S8_-19_F | GTTAAAAAAWCCTTGAGTCATG |
| 9 | BTV_S9_-15_F | GTTAAAAAATCGCATATGTCAG |
| 10 | BTV_S10_F | GTTAAAAAGTGTCGCTGCCAT |
| Reverse primer |
||
| 2 | BTV4&20_S2_R | GTAAGTGTAAGAAGGCCACAG |
| 5 | BTV_S5_R | GTAAGTTGAAAAGTTCTAGTAGAG |
| 6 | BTV4&10_S6_1618_R | GTAAGTGTAATCTTCTCCCTC |
| 7 | BTV_S7_1142_R | GTAAGTGTAATCTAAGAGACGT |
| 8 | BTV_S8_1106_R | GTAAGTGTAAAATCCCCCCC |
| 9 | BTV_S9_1038_R | GTAAGTRTGAAATCGCCCTAC |
| 10 | BTV_S10_R | ACCTYGGGGCGCCACTC |
*BTV, bluetongue virus.
