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Volume 14, Number 2—February 2008

Dispatch

Experimental Infection and Natural Contact Exposure of Dogs with Avian Influenza Virus (H5N1)

Matthias Giese*, Timm C. Harder*, Jens P. Teifke*, Robert Klopfleisch*, Angele Breithaupt*, Thomas C. Mettenleiter*, and Thomas W. Vahlenkamp*Comments to Author 
Author affiliations: *Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany;

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Table

Course of experimental influenza virus (H5N1) infection of dogs and in-contact animals*

Dog no. EID50 Body temperature (dpi)
Pharyngeal swab (dpi)
Euthanized
(dpi) Antibodies
0 2 4 6 0 2 4 6 HI ELISA
1 106 38.5 39.2 38.8 38.8 + 10 (+)†
2 106 38.4 39.7 39.1 38.8 + 10 (+)‡
3 106 38.5 39.3 38.6 38.4 + 21 16 +
4 106 38.7 39.3 39.1 38.6 21
5 (dog contact) 0 38.3 38.4 38.0 38.7 21
6 (cat contact) 0 38.4 38.2 38.4 38.5 21
7 (cat contact) 0 38.3 38.5 38.6 38.8 21
8 (cat contact) 0 38.4 38.5 38.2 38.3 21
9 (neg. control) 0 38.4 38.2 38.7 37.8 21
10 (neg. control) 0 38.3 38.6 38.8 38.0 21

*Elevated body temperatures (above 39°C) are underlined. Real time reverse transcription–PCR results are given as – (cycle threshold [ct] values >38) and + (ct values 31–38). Animal serum samples were examined by hemagglutination-inhibition (HI) using highly pathogenic influenza virus (H5N1) strain A/cat/Germany/R606/2006 (9) as antigen. Results are given as reciprocal titers. Competitive nucleoprotein ELISA results are given as – (inhibition <65%) and + (inhibition >65); EID50, 50% egg infectious dose; dpi, days postinfection.
†Serum from dog no. 1 on dpi 10 showed 39.4% inhibition.
‡Serum from dog no. 2 showed 21.6% inhibition.

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