Hemagglutinating Encephalomyelitis Coronavirus Infection in Pigs, Argentina
Maria A. Quiroga* , Javier Cappuccio*, Pablo Piñeyro*, Walter Basso*, Gastón Moré*, Mariana Kienast†, Sergio Schonfeld‡, José L. Cáncer‡, Sandra Arauz*, María E. Pintos*, Mariana Nanni†, Mariana Machuca*, Norio Hirano§, and Carlos J. Perfumo*
Author affiliations: *Universidad Nacional de la Plata, La Plata, Argentina; †Centro de Investigaciones en Ciencias Veterinarias, Castelar, Argentina; ‡Private practice, Roque Perez, Argentina; §Iwate University, Morioka, Japan;
Figure 2. Polyacrylamide gel and silver staining of reverse transcription–PCR products from brains of piglets infected with porcine hemagglutinating encephalomyelitis coronavirus. Amplicons of ≈250 bp were found in brain samples from pigs 6, 8, 9, and 11 days of age. Neg, negative control (water + mastermix); PK15, amplification of PK15 cells inoculated with brain and tonsil from affected piglet; AP, asymptomatic piglet; and Ladder, 50-bp Fermentas.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.