Volume 14, Number 3—March 2008
Research
Hantavirus RNA in Saliva from Patients with Hemorrhagic Fever with Renal Syndrome
Lisa Pettersson*, Jonas Klingström*†‡, Jonas Hardestam†‡, Åke Lundkvist†‡, Clas Ahlm*, and Magnus Evander*
Table
Comparison between detection of PUUV RNA in saliva and plasma and antibodies in serum samples from 14 patients with nephropathia epidemica*
| Patient no. | Sex | Age, y | Date saliva collected (d of disease) | PUUV RNA† in saliva, copies/mL | PUUV RNA† in plasma,‡ copies/mL | PUUV IgM§ in serum‡ | PUUV IgG titer in serum‡ |
|---|---|---|---|---|---|---|---|
| 1 | M | 65 | 6 | 121,323 | 959,294 | + | 40 |
| 2 | M | 64 | 2 | 66,994 | 117,562 | + | 80 |
| 3 | M | 33 | 5 | 44,898 | 40,427 | + | >640 |
| 4 | F | 38 | 6 | 17,516 | 1,381,413 | + | 40 |
| 5 | F | 59 | 9 | 9,582 | 3,724 | + | >640 |
| 6 | M | 54 | 5 | 6,372 | 26,626 | – | 320 |
| 7 | M | 39 | 7 | 3,745 | 189,233 | + | 320 |
| 8 | M | 28 | 5 | 2,589 | 36,231 | + | >640 |
| 9 | F | 26 | 5 | 2,163 | 54,315 | + | 80 |
| 10 | F | 39 | 5 | 1,530 | 3,044 | + | 80 |
| 11 | M | 38 | 6 | 0 | 1,952 | ± | >640 |
| 12 | M | 41 | 6 | 0 | 5,215 | + | 40 |
| 13 | F | 57 | 2 | 0 | 41,271 | + | 320 |
| 14 | F | 62 | 7 | 0 | 81,330 | + | 160 |
*PUUV, Puumala virus; Ig, immunoglobulin.
†Detected by real-time reverse transcription–PCR.
‡Most plasma and serum samples were collected the same day or within 1 day before or after the saliva sample and antibody was detected by immunofluorescence assay.
§Serum was diluted 1:16 and scored as negative (–), positive (+), or weakly positive (±).
