Rapid Typing of Transmissible Spongiform Encephalopathy Strains with Differential ELISA
Stéphanie Simon*, Jérôme Nugier*, Nathalie Morel*, Hervé Boutal*, Christophe Créminon*, Sylvie L. Benestad†, Olivier Andréoletti‡, Frédéric Lantier§, Jean-Marc Bilheude¶, Muriel Feyssaguet¶, Anne-Gaëlle Biacabe#, Thierry Baron#, and Jacques Grassi*
Author affiliations: *Commissariat à l’Energie Atomique, Gif-sur-Yvette, France; †National Veterinary Institute, Oslo, Norway; ‡Institut National de la Recherche Agronomique-Ecole Nationale Vétérinaire de Toulouse, Toulouse, France; §Infectiologie Animale et Santé Publique, Tours, France; ¶Bio-Rad, Marnes-la-Coquette, France; #Agence Française de Sécurité Sanitaire des Aliments, Lyon, France;
Figure 3. A total of 260 French field-positive isolates were analyzed with the automated ELISA typing test. Each series of 29 samples was analyzed together with 3 internal controls (classic scrapie, intermediate scrapie, experimental ovine bovine spongiform encephalopathy [BSE]). The ratio obtained for each sample was normalized by dividing by the experimental ovine BSE in the same series. Open bars represent the atypical scrapie cases. Natural goat BSE isolate (Ch636) is indicated.
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