Rapid Typing of Transmissible Spongiform Encephalopathy Strains with Differential ELISA
Stéphanie Simon*, Jérôme Nugier*, Nathalie Morel*, Hervé Boutal*, Christophe Créminon*, Sylvie L. Benestad†, Olivier Andréoletti‡, Frédéric Lantier§, Jean-Marc Bilheude¶, Muriel Feyssaguet¶, Anne-Gaëlle Biacabe#, Thierry Baron#, and Jacques Grassi*
Author affiliations: *Commissariat à l’Energie Atomique, Gif-sur-Yvette, France; †National Veterinary Institute, Oslo, Norway; ‡Institut National de la Recherche Agronomique-Ecole Nationale Vétérinaire de Toulouse, Toulouse, France; §Infectiologie Animale et Santé Publique, Tours, France; ¶Bio-Rad, Marnes-la-Coquette, France; #Agence Française de Sécurité Sanitaire des Aliments, Lyon, France;
Figure 4. Analysis of goats isolates with ELISA typing test and immunoblot. Two experimental bovine spongiform encephalopathy (BSE) samples in goats analyzed by using the manual typing ELISA (A) gave ratios similar to those of experimental BSE in sheep. Results are the mean of 3 independent experiments. Experimental goat BSE and BSE-like field isolate Ch636 were analyzed by Western blot and compared with scrapie goat isolates (B). Lane 1, untreated negative brain homogenate. Lane 2, proteinase K (PK)–treated negative brain homogenate. Lanes 3–9, PK-treated positive isolates: French scrapie isolate 99–1316 (lane 3); experimental ovine BSE 397 BS (lane 4); experimental BSE goat 2 (lane 5); French goat isolate Ch 636 (campaign 2002) (lane 6); French scrapie goat isolates Ch517 and Ch519 (campaign 2002) (lanes 7 and 8, respectively); Norwegian scrapie isolate (Lavik, lane 9). MW, molecular weight.
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