Volume 14, Number 4—April 2008
Research
Rapid Typing of Transmissible Spongiform Encephalopathy Strains with Differential ELISA
Stéphanie Simon*, Jérôme Nugier*, Nathalie Morel*, Hervé Boutal*, Christophe Créminon*, Sylvie L. Benestad†, Olivier Andréoletti‡, Frédéric Lantier§, Jean-Marc Bilheude¶, Muriel Feyssaguet¶, Anne-Gaëlle Biacabe#, Thierry Baron#, and Jacques Grassi*
Table 1
Analysis of the reproducibility of the ELISA typing test*
| Sample (N = 20) | Mean ratio A/A′(normalized) | SD (normalized) | CV, % | 95% CI (mean ± 2× SD) |
|---|---|---|---|---|
| Manual protocol | ||||
| Classic scrapie 99-1316 | 1.3 (0.13) | 0.2 (0.02) | 15.4 | 0.9–1.7 (0.09–0.18) |
| Intermediate scrapie PG1259 | 4.5 (0.46) | 0.8 (0.08) | 17.8 | 2.9–6.1 (0.30–0.63) |
| Experimental ovine BSE 397BS | 9.7 (1.00) | 2.0 (0.21) | 20.6 | 5.7–13.7 (0.59–1.41) |
| Automated protocol | ||||
| Classic scrapie 99-1316 | 1.6 (0.25) | 0.2 (0.03) | 13.7 | 1.2–1.9 (0.19–0.30) |
| Intermediate scrapie 99-1487 | 3.8 (0.59) | 0.5 (0.08) | 13.4 | 2.8–4.8 (0.44–0.75) |
| Experimental ovine BSE 397BS | 6.4 (1.00) | 0.9 (0.14) | 14.8 | 4.5–8.3 (0.70–1.30) |
*Three samples (classic scrapie 99-1316, intermediate scrapie PG1259 or 99-1487, and experimental ovine BSE 397BS) were analyzed in 20 different experiments using the manual or automated protocol (see Experimental Procedures). Normalized ratios are indicated in parentheses. SD, standard deviation; CV, coefficient of variation; CI, confidence interval; BSE, bovine spongiform encephalopathy.
