Volume 14, Number 4—April 2008
Research
Rapid Typing of Transmissible Spongiform Encephalopathy Strains with Differential ELISA
Stéphanie Simon*, Jérôme Nugier*, Nathalie Morel*, Hervé Boutal*, Christophe Créminon*, Sylvie L. Benestad†, Olivier Andréoletti‡, Frédéric Lantier§, Jean-Marc Bilheude¶, Muriel Feyssaguet¶, Anne-Gaëlle Biacabe#, Thierry Baron#, and Jacques Grassi*
Table 2
Analysis of the experimental caprine BSE samples using the manual ELISA typing test*
| Samples | Mean A/A′ratio (normalized) | SD (normalized) | CV, % | 95% CI (mean ± 2×SD) |
|---|---|---|---|---|
| Experimental BSE goat 1 | 8.2 (0.75) | 1.0 (0.09) | 12.2 | 6.2–10.2 (0.57–0.93) |
| Experimental BSE goat 2 | 9.1 (0.83) | 1.1 (0.10) | 12.1 | 6.9–11.3 (0.63–1.04) |
| 99-1316 | 1.5 (0.14) | 0.2 (0.02) | 13.3 | 1.1–1.9 (0.10–0.17) |
| PG1259 | 4.1 (0.38) | 0.6 (0.06) | 14.6 | 2.9–5.3 (0.27–0.49) |
| Experimental ovine BSE 397BS | 10.9 (1.00) | 1.8 (0.17) | 12.2 | 7.3–14.5 (0.67–1.33) |
*BSE, bovine spongiform encephalopathy; SD, standard deviation; CV, coefficient of variation; CI, confidence interval.
