Rapid Typing of Transmissible Spongiform Encephalopathy Strains with Differential ELISA
Stéphanie Simon*, Jérôme Nugier*, Nathalie Morel*, Hervé Boutal*, Christophe Créminon*, Sylvie L. Benestad†, Olivier Andréoletti‡, Frédéric Lantier§, Jean-Marc Bilheude¶, Muriel Feyssaguet¶, Anne-Gaëlle Biacabe#, Thierry Baron#, and Jacques Grassi*
Author affiliations: *Commissariat à l’Energie Atomique, Gif-sur-Yvette, France; †National Veterinary Institute, Oslo, Norway; ‡Institut National de la Recherche Agronomique-Ecole Nationale Vétérinaire de Toulouse, Toulouse, France; §Infectiologie Animale et Santé Publique, Tours, France; ¶Bio-Rad, Marnes-la-Coquette, France; #Agence Française de Sécurité Sanitaire des Aliments, Lyon, France;
Appendix Figure 1. Sensitivity of the ELISA typing test. A) ARQ/ARQ genotype. Fifteen experimental ovine bovine spongiform encephalopathy (BSE) animals from a first passage and 10 from a second passage were tested by using the typing ELISA, in at least 3 independent experiments. For some animals, different regions of the central nervous system were tested (animals SB1, 359, 397, 7704, and 7705). SC, spinal cord. BS, brain stem; FC, frontal cortex; PC, parietal cortex; OC, occipital cortex. B) ARR/ARR genotype. Seven experimental ovine BSE animals from a first passage were tested by using the typing ELISA, in at least 4 independent experiments.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.