Emerging Infectious Disease ISSN: 1080-6059

Rapid Typing of Transmissible Spongiform Encephalopathy Strains with Differential ELISA

Stéphanie Simon*, Jérôme Nugier*, Nathalie Morel*, Hervé Boutal*, Christophe Créminon*, Sylvie L. Benestad†, Olivier Andréoletti‡, Frédéric Lantier§, Jean-Marc Bilheude¶, Muriel Feyssaguet¶, Anne-Gaëlle Biacabe#, Thierry Baron#, and Jacques Grassi*

Author affiliations: *Commissariat à l’Energie Atomique, Gif-sur-Yvette, France; †National Veterinary Institute, Oslo, Norway; ‡Institut National de la Recherche Agronomique-Ecole Nationale Vétérinaire de Toulouse, Toulouse, France; §Infectiologie Animale et Santé Publique, Tours, France; ¶Bio-Rad, Marnes-la-Coquette, France; #Agence Française de Sécurité Sanitaire des Aliments, Lyon, France;

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Appendix Figure 2

Proteinase K (PK) sensitivity of 21 Norwegian isolates in mild conditions. The ELISA typing test was performed on 20 Nor98 isolates and 1 Norwegian scrapie isolate (Lavik) by using the protective A´´ reagent, with a PK concentration of 1.2 µg/mg tissue. These new conditions (PK 1.2 µg/mg of tissue in A´´ reagent) showed a large range of PK resistance with normalized ratios varying from 1.4 for the most resistant (Lindas isolate), close to the experimental ovine BSE, to >14 for the weakest (Soknedal2). One isolate, Tennevoll, appeared so sensitive to PK digestion that the ratio could not be evaluated (>95) even using these protective conditions.

Appendix Figure 2. Proteinase K (PK) sensitivity of 21 Norwegian isolates in mild conditions. The ELISA typing test was performed on 20 Nor98 isolates and 1 Norwegian scrapie isolate (Lavik) by using the protective A´´ reagent, with a PK concentration of 1.2 µg/mg tissue. These new conditions (PK 1.2 µg/mg of tissue in A´´ reagent) showed a large range of PK resistance with normalized ratios varying from 1.4 for the most resistant (Lindas isolate), close to the experimental ovine BSE, to >14 for the weakest (Soknedal2). One isolate, Tennevoll, appeared so sensitive to PK digestion that the ratio could not be evaluated (>95) even using these protective conditions.

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