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Volume 14, Number 5—May 2008

Dispatch

Naturally Acquired Human Plasmodium knowlesi Infection, Singapore

Lee Ching Ng§Comments to Author , Eng Eong Ooi†, Cheng Chuan Lee*, Piao Jarrod Lee‡, Oon Tek Ng*Comments to Author , Sze Wong Pei§, Tian Ming Tu‡, Jin Phang Loh†, and Yee Sin Leo*
Author affiliations: *Tan Tock Seng Hospital, Singapore; †DSO National Laboratories, Singapore; ‡Singapore Armed Forces Headquarters Medical Corps, Singapore; §Environmental Health Institute, Singapore;

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Table

Primers used for the PCR investigation of the clinical sample from Singapore*

Primers Forward Sequence (5′ → 3’) Reverse Sequence (5′ → 3’) Results
Nest 1, genus specific primers
Genus specific (11)
rPLU 1
TCA AAG AAT AAG CCA TGC AAG TGA
rPLU 2
TAC CCT GTT GTT GCC TTA AAC TCC
+
Nest 2, genus- and species-specific primers
Genus specific (11) rPLU 3 TTT TTA TAA GGA TAA CTA CGG AAA AGC TGT rPLU 4 TAC CCG TCA TAG CCA TGT TAG GCC AAT ACC +
Plasmodium knowlesi 
 specific (5) Pmk8 GTT AGC GAG AGC CAC AAA AAA GCG AAT Pmkr9 ACT CAA AGT AAC AAA ATC TTC CGT A +
P. vivax specific (11) rVIV1 CGC TTC TAG CTT AAT CCA CAT AAC TGA TAC rVIV2 ACT TCC AAG CCG AAG CAA AGA AAG TCC TTA
P. falciparum specific (11) rFAL1 TTA AAC TGG TTT GGG AAA ACC AAA TAT ATT rFAL2 ACA CAA TGA ACT CAA TCA TGA CTA CCC GTC
P. malariae specific (11) rMAL1 ATA ACA TAG TTG TAC GTT AAG AAT AAC CGC rMAL2 AAA ATT CCC ATG CAT AAA AAA TTA TAC AAA
P. ovale specific (11) rOVA1 ATC TCT TTT GCT ATT TTT TAG TAT TGG AGA rOVA2 GGA AAA GGA CAC ATT AAT TGT ATC CTA GTG

*PCR was carried out at the Environmental Health Institute and cycling conditions used were as described in the references shown in parentheses.
+, positive; –,negative.

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