Emerging Infectious Disease ISSN: 1080-6059

Fatal Rickettsia conorii subsp. israelensis Infection, Israel

Miriam Weinberger*†

, Avi Keysary‡, Judith Sandbank*, Ronit Zaidenstein*, Avi Itzhaki*, Carmela Strenger‡, Moshe Leitner‡, Christopher D. Paddock§, and Marina E. Eremeeva§

Author affiliations: *Assaf Harofeh Medical Center, Zerifin, Israel; †Tel Aviv University, Ramat Aviv, Israel; ‡Israel Institute for Biological Research, Ness-Ziona, Israel; §Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

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Figure 2

PCR product from the 17-kDa protein antigen gene obtained from DNA extracted from necropsied tissues of the patient. Primary PCR (A), nested PCR (B), and BfaI restriction enzyme pattern of the 17-kDa protein gene amplicon (C). Lane 1, reagent control; 2, skin; 3, liver; 4, lung; NTC, nontemplate control; SF, Rickettsia conorii DNA control; SM, size markers.

Figure 2. PCR product from the 17-kDa protein antigen gene obtained from DNA extracted from necropsied tissues of the patient. Primary PCR (A), nested PCR (B), and BfaI restriction enzyme pattern of the 17-kDa protein gene amplicon (C). Lane 1, reagent control; 2, skin; 3, liver; 4, lung; NTC, nontemplate control; SF, Rickettsia conorii DNA control; SM, size markers.

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