Emerging Infectious Disease ISSN: 1080-6059

Cryptococcus neoformans Strains and Infection in Apparently Immunocompetent Patients, China

Jianghan Chen*, Ashok Varma†, Mara R. Diaz‡, Anastasia P. Litvintseva§, Kurt K. Wollenberg†, and Kyung J. Kwon-Chung†

Author affiliations: *Shanghai Changzheng Hospital, Shanghai, People’s Republic of China; †National Institutes of Health, Bethesda, Maryland, USA; ‡University of Miami, Miami, Florida, USA; §Duke University Medical Center, Durham, North Carolina, USA;

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Appendix Figure 2

Phylogenetic tree constructed on the partial sequence of intergenic spacer region 1 (IGS)1-5.8S-IGSII region. The tree was computed with PAUP*4 (24) (heuristic search, stepwise addition, random addition sequence, nearest neighbor interchange, 100 maximum trees). Numbers represent bootstrap values of 500 replicates. Sequence data contained a total of 770 characters (747 constant characters; 4 uninformative characters; 19 parsimony informative characters). Gaps were represented as missing data. Each character was treated as an independent, unordered, multiple character of equal weight.

Appendix Figure 2. Phylogenetic tree constructed on the partial sequence of intergenic spacer region 1 (IGS)1-5.8S-IGSII region. The tree was computed with PAUP*4 (24) (heuristic search, stepwise addition, random addition sequence, nearest neighbor interchange, 100 maximum trees). Numbers represent bootstrap values of 500 replicates. Sequence data contained a total of 770 characters (747 constant characters; 4 uninformative characters; 19 parsimony informative characters). Gaps were represented as missing data. Each character was treated as an independent, unordered, multiple character of equal weight.

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